Mosquito (Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes.
Autor(es): Duchesne Laurence; Hubert Jean-François; Verbavatz Jean-Marc; Thomas Daniel; Pietrantonio Patricia V
Resumo: Previous results showed that mRNA encoding a putative aquaporin (AQP) (GenBank accession number AF218314) is present in the tracheolar cells associated with female Aedes aegypti Malpighian tubules. In this study, immunohistochemistry detected the protein, AeaAQP, also in tracheolar cells, suggesting its involvement in water movement in the respiratory system. When expressed in Xenopus oocytes, AeaAQP increased the osmotic water permeability from 15 x 10(-6) to 150 x 10(-6) m x s-1, which was inhibited by mercury ions. No permeability to glycerol or other solute was observed. AeaAQP expressed in oocytes was solubilized as a homotetramer in nondenaturing detergent as deduced from velocity centrifugation on density gradients. Phylogenetic analysis of MIP (major intrinsic protein) family sequences shows that AeaAQP clusters with other native orthogonal array forming proteins. Specific orthogonal arrays were detected by freeze-fracture analysis of AeaAQP oocyte membranes. We conclude that, in tracheolar cells of A. aegypti, AeaAQP is probably a highly water-permeable homotetrameric MIP which natively can form 2D crystals.
Palavras-Chave: Aquaporin; Insect respiration; Malpighian tubule; Tracheolar cell tracheoles
Imprenta: European Journal of Biochemistry / FEBS, v. 270, n. 3, p. 422-429, 2003
Identificador do objeto digital: 10.1046/j.1432-1033.2003.03389.x
Descritores: Aedes aegypti - Cell ; Aedes aegypti - DNA ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA
Data de publicação: 2003