Microsatellite markers for population genetic studies in Aedes aegypti (Diptera: Culicidae) from C?´te d'Ivoire: evidence for a microgeographic genetic differentiation of mosquitoes from Bouak??.
Autor(es)Ravel S; Herv?? J-P; Diarrassouba S; Kone A; Cuny G
ResumoIn West Africa, Aedes aegypti (Diptera: Culicidae) (Linnaeus, C., 1762. Zweyter Theil, enhalt Beschreibungen veschiedener wichtiger Naturalien. In: Hasselquist, F. (Ed.), Reise nach Palastina in den Jahren von 1749 bis 1752, Rostock, Germany, pp. 267-606) represents the principal vector of yellow fever. This study reports the use of microsatellite markers to characterise various A. aegypti populations from C?´te d'Ivoire according to a north-south transect, and to perform a temporal genetic survey of the mosquitoes. Three microsatellite loci were used to analyse individuals from four different places: Kabolo, Bouak??, and two different districts of Abidjan. We found that the four populations are genetically distinct except the two Abidjan populations. In the Bouak?? population, the coexistence of two cryptic species, not morphologically distinguishable, seems to account for the extensive heterozygote deficiency observed. Comparison of mosquitoes from Bouak?? 1 year apart indicated that a dramatic change occurred in the structuring of this population over time. Taken together these results indicate that microsatellite markers could be useful for identifying various populations of A. aegypti on a microgeographic scale and to assess for temporal variation within mosquito populations.
Palavras-ChaveAedes aegypti; Yellow fever; Microsatellites; Polymorphism
ImprentaActa Tropica, v. 82, n. 1, p. 39-49, 2002
Identificador do Objeto Digital10.1016/S0001-706X(02)00028-1
DescritoresAedes aegypti - Pathogenesis ; Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Epidemiology
Data de Publicação:2002