Macrophage migration inhibitory factor is necessary for the lipo-oligosaccharide-induced response by modulation of Toll-like receptor 4 in monocytes from GBS patients.
Autor(es): Wang Yu-Zhong; Tian Fa-Fa; Liu Hao; Zhang Wei,Li Jing; Xiao Bo; Zhou Wen-Bin
Resumo: Previous studies have suggested that macrophage migration inhibitory factor (MIF) may play a critical role in the pathogenesis of Guillain-Barre syndrome (GBS); however, its definite mechanism remains unknown. In this study, we prepared the monocytes from the peripheral blood mononuclear cells (PBMCs) of GBS patients and the controls. Lipo-oligosaccharide (LOS) from Campylobacter jejuni was used as the stimulus of the monocytes in vitro and siRNA-MIF was used to explore the roles of MIF in LOS-induced response. Significantly, silencing of MIF attenuated the LOS-induced up-regulation of Toll-like receptor 4 (TLR4) and translocation of NF-?B into the nucleus; we also observed the up-regulation of IL-12p40, TNF-?, IL-6, CXCL8 and CCL5 in GBS monocytes with LOS stimulus; and siRNA-MIF overrided the effects of LOS on the production of the TNF-?, IL-6, and CXCL8. Conclusively, our study provides evidences that MIF may participate in the pathogenesis of GBS by modulating the LOS-induced response through TLR4 signaling pathway.
Imprenta: Journal of Neuroimmunology, v. 257, n. 1-2, p. 67-75, 2013
Identificador do objeto digital: 10.1016/j.jneuroim.2013.01.006
Descritores: Guillain-Barre Syndrome - Biosynthesis ; Guillain-Barre Syndrome - Cell ; Guillain-Barre Syndrome - Cytopathology ; Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - Cytokines ; Guillain-Barre Syndrome - Immunology
Data de publicação: 2013
