Proteome analysis of cerebrospinal fluid in Guillain-Barré syndrome (GBS).
Autor(es): Lehmensiek V.; Süssmuth S D.; Brettschneider J.; Tauscher G.; Felk S.; Gillardon F.; Tumani H.
Resumo: We used two-dimensional difference in-gel electrophoresis (2-D-DIGE) for proteome analysis of cerebrospinal fluid (CSF) in Guillain-Barré syndrome (GBS). Spots showing >2-fold difference between GBS and controls were analysed using MALDI-TOF mass spectrometry. Proteins that were up-regulated in GBS included haptoglobin, serine/threonine kinase 10, alpha-1-antitrypsin, SNC73, alpha II spectrin, IgG kappa chain and cathepsin D preprotein, while transferrin, caldesmon, GALT, human heat shock protein 70, amyloidosis patient HL-heart-peptide 127aa and transthyretin were down-regulated. Some of these proteins are reported in CSF of GBS for the first time. Accordingly, the 2-D-DIGE technology may be useful to identify disease-specific proteins in patients with GBS.
Imprenta: Journal of Neuroimmunology, v. 185, n. 1-2, p. 190-194, 2007
Identificador do objeto digital: 10.1016/j.jneuroim.2007.01.022
Descritores: Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - Proteome
Data de publicação: 2007
