Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides.
Autor(es): Moyano Ana L.; Comín Romina.; Vilcaes Aldo A.; Funes Samanta C.; Roth Germán A.; Irazoqui Fernando J.; Nores Gustavo A.
Resumo: Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barré syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed density-dependent" binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes)."
Imprenta: Glycobiology, v. 22, n. 12, p. 1768-1774, 2012
Identificador do objeto digital: 10.1093/glycob/cws117
Descritores: Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - Antibodies ; Guillain-Barre Syndrome - Immunology
Data de publicação: 2012
