Substitution of wild-type yellow fever Asibi sequences for 17D vaccine sequences in ChimeriVax-dengue 4 does not enhance infection of Aedes aegypti mosquitoes
Autor(es): McGee Charles E,Tsetsarkin Konstantin,Vanlandingham Dana L,McElroy Kate L,Lang Jean,Guy Bruno,Decelle Thierry,Higgs Stephen
Resumo: To address concerns that a flavivirus vaccine/wild-type recombinant virus might have a high mosquito infectivity phenotype, the yellow fever virus (YFV) 17D backbone of the ChimeriVax-dengue 4 virus was replaced with the corresponding gene sequences of the virulent YFV Asibi strain. Field-collected and laboratory-colonized Aedes aegypti mosquitoes were fed on blood containing each of the viruses under investigation and held for 14 days after infection. Infection and dissemination rates were based on antigen detection in titrated body or head triturates. Our data indicate that, even in the highly unlikely event of recombination or substantial backbone reversion, virulent sequences do not enhance the transmissibility of ChimeriVax viruses. In light of the low-level viremias that have been observed after vaccination in human volunteers coupled with low mosquito infectivity, it is predicted that the risk of mosquito infection and transmission of ChimeriVax vaccine recombinant/revertant viruses in nature is minimal.
Imprenta: The Journal of infectious diseases, v. 197, n. 5, p. 686-692, 2008
Identificador do objeto digital: 10.1086/527328
Descritores: Aedes aegypti - Cytopathology ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Inflammation ; Aedes aegypti - Viral infections ; Aedes aegypti - virus ; Aedes aegypti - Transmission ; Aedes aegypti - Vaccine ; Aedes aegypti - Dengue ; Aedes aegypti - Immunology ; Aedes aegypti - Public health
Data de publicação: 2008