SSCP analysis of scnDNA for genetic profiling of Aedes aegypti
Autor(es): Wong Jacklyn,Tripet Frédéric,Rasgon Jason L,Lanzaro Gregory C,Scott Thomas W
Resumo: We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 79, n. 4, p. 511-517, 2008
Identificador do objeto digital: 10.4269/ajtmh.2008.79.511
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis
Data de publicação: 2008
