Transmission-blocking antibodies against mosquito C-type lectins for dengue prevention
Autor(es): Liu Yang,Zhang Fuchun,Liu Jianying,Xiao Xiaoping,Zhang Siyin,Qin Chengfeng,Xiang Ye,Wang Penghua,Cheng Gong
Resumo: C-type lectins are a family of proteins with carbohydrate-binding activity. Several C-type lectins in mammals or arthropods are employed as receptors or attachment factors to facilitate flavivirus invasion. We previously identified a C-type lectin in Aedes aegypti, designated as mosquito galactose specific C-type lectin-1 (mosGCTL-1), facilitating the attachment of West Nile virus (WNV) on the cell membrane. Here, we first identified that 9 A. aegypti mosGCTL genes were key susceptibility factors facilitating DENV-2 infection, of which mosGCTL-3 exhibited the most significant effect. We found that mosGCTL-3 was induced in mosquito tissues with DENV-2 infection, and that the protein interacted with DENV-2 surface envelop (E) protein and virions in vitro and in vivo. In addition, the other identified mosGCTLs interacted with the DENV-2 E protein, indicating that DENV may employ multiple mosGCTLs as ligands to promote the infection of vectors. The vectorial susceptibility factors that facilitate pathogen invasion may potentially be explored as a target to disrupt the acquisition of microbes from the vertebrate host. Indeed, membrane blood feeding of antisera against mosGCTLs dramatically reduced mosquito infective ratio. Hence, the immunization against mosGCTLs is a feasible approach for preventing dengue infection. Our study provides a future avenue for developing a transmission-blocking vaccine that interrupts the life cycle of dengue virus and reduces disease burden.
Imprenta: PLoS Pathogens, v. 10, n. 2, p. e1003931, 2014
Identificador do objeto digital: 10.1371/journal.ppat.1003931
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - Antibodies ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Viral infections ; Aedes aegypti - RT-PCR ; Aedes aegypti - virus ; Aedes aegypti - Transmission ; Aedes aegypti - Vaccine ; Aedes aegypti - Dengue ; Aedes aegypti - Epidemiology ; Aedes aegypti - Immunology ; Aedes aegypti - Public health
Data de publicação: 2014
