Use of Centrifugal Filter Devices to Concentrate Dengue Virus in Mosquito per os Infection Experiments
Autor(es)Richard Vaea, Viallon Jérôme, Cao-Lormeau Van-Mai
ResumoDengue virus (DENV) is an arbovirus transmitted to humans by the bite of infected Aedes mosquitoes. Experimental per os infection of mosquitoes with DENV is usually a preliminary step in virus/vector studies but it requires being able to prepare artificial blood-meals with high virus titers. We report here the convenient use of centrifugal filter devices to quickly concentrate DENV particles in cell-culture supernatants. The median viral titer in concentrated-supernatants was 8.50 log10 TCID50/mL. By using these DENV concentrated-supernatants to prepare infectious blood-meals in Aedes aegypti per os infection experiments, we obtained a mean mosquito-infection rate of 94%. We also evaluated the use of centrifugal filter devices to recover DENV particles from non-infectious blood-meals presented to infected mosquitoes through a feeding membrane to collect their saliva.
ImprentaPloS One, v. 10, n. 9, p. e0138161, 2015
Identificador do Objeto Digital10.1371/journal.pone.0138161
DescritoresAedes aegypti - Arbovirus ; Aedes aegypti - Cell ; Aedes aegypti - virus ; Aedes aegypti - Dengue
Data de Publicação:2015
