Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth
Autor(es): Haghighat-Khah Roya Elaine,Scaife Sarah,Martins Sara,St John Oliver,Matzen Kelly Jean,Morrison Neil,Alphey Luke
Resumo: Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE) mechanisms. We successfully demonstrated the established ?C31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ?C31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests.
Imprenta: PloS One, v. 10, n. 4, p. e0121097, 2015
Identificador do objeto digital: 10.1371/journal.pone.0121097
Descritores: Aedes aegypti - Genome ; Aedes aegypti - Public health
Data de publicação: 2015
