The use of direct sequencing of dengue virus cDNA from individual field-collected Aedes aegypti for surveillance and epidemiological studies
Autor(es): Romero-Vivas C M, Sutherland C J, Falconar A K
Resumo: The relative efficiencies of four methods to extract viral RNA from individual dengue-2 virus (D-2V)-infected mosquitoes, Aedes aegypti (L.) (Diptera: Culicidae), were compared. The most efficient of these methods was then used to extract viral RNA for the preparation of cDNA from the abdomens of six engorged D-2V-infected mosquitoes and sera from three dengue fever (DF) patients collected in an isolated rural town in Colombia. Comparisons of viral envelope (E) gene sequences from each of these strongly suggested that the D-2V population which circulated in this study area was a homogeneous genotype which was unrelated to any of the D-2 viruses isolated from elsewhere in the world. When coupled with our rapid method to identify viruses in individual mosquitoes (Romero-Vivas et al. (1998) Medical and Veterinary Entomology, 12, 101-105), the methodology we describe should be useful for epidemiological and surveillance studies of dengue viruses and other arboviruses.
Palavras-Chave: Aedes aegypti; cDNA; Dengue virus; Immunofluorescence assay; Polymerase chain reaction; Sequencing; Colombia
Imprenta: Medical and Veterinary Entomology, v. 14, n. 1, p. 89-94, 2000
Identificador do objeto digital: 10.1046/j.1365-2915.2000.00218.x
Descritores: Aedes aegypti - Arbovirus ; Aedes aegypti - DNA ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Viral infections ; Aedes aegypti - Molecular methods ; Aedes aegypti - virus ; Aedes aegypti - Dengue ; Aedes aegypti - Epidemiology ; Aedes aegypti - Public health
Data de publicação: 2000
