Rapid determination of viral RNA sequences in mosquitoes collected in the field
Autor(es): Kihara Yuki,Satho Tomomitsu,Eshita Yuki,Sakai Kouji,Kotaki Akira,Takasaki Tomohiko,Rongsriyam Yupha,Komalamisra Narumon,Srisawat Raweewan,Lapcharoen Parichat,Sumroiphon Suchada,Iwanaga Shiroh,Ushijima Hiroshi,Endoh Daiji,Miyata Takeshi,Sakata Akira,Kashige Nobuhiro,Miake Fumio,Fukushi Shuetsu,Saijo Masayuki,Kurane Ichiro,Morikawa Shigeru,Mizutani Tetsuya
Resumo: A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses.
Palavras-Chave: Rapid determination; Dengue virus type 4; Cell fusing agent virus; Aedes aegypti
Imprenta: Journal of Virological Methods, v. 146, n. 1-2, p. 372-374, 2007
Identificador do objeto digital: 10.1016/j.jviromet.2007.07.008
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Viral infections ; Aedes aegypti - RT-PCR ; Aedes aegypti - virus ; Aedes aegypti - Dengue
Data de publicação: 2007
