Targeted release and fractionation reveal glucuronylated and sulphated N- and O-glycans in larvae of dipteran insects
Autor(es): Kurz Simone,Aoki Kazuhiro,Jin Chunsheng,Karlsson Niclas G,Tiemeyer Michael,Wilson Iain B H,Paschinger Katharina
Resumo: Mosquitoes are important vectors of parasitic and viral diseases with Anopheles gambiae transmitting malaria and Aedes aegypti spreading yellow and Dengue fevers. Using two different approaches (solid-phase extraction and reversed-phase or hydrophilic interaction HPLC fractionation followed by MALDI-TOF MS or permethylation followed by NSI-MS), we examined the N-glycans of both A. gambiae and A. aegypti larvae and demonstrate the presence of a range of paucimannosidic glycans as well as bi- and tri-antennary glycans, some of which are modified with fucose or with sulphate or glucuronic acid residues; the latter anionic modifications were also found on N-glycans of larvae from another dipteran species (Drosophila melanogaster). The sulphate groups are attached primarily to core α-mannose residues (especially the α1,6-linked mannose), whereas the glucuronic acid residues are linked to non-reducing β1,3-galactose. Also, O-glycans were found to possess glucuronic acid and sulphate as well as phosphoethanolamine modifications. The presence of sulphated and glucuronylated N-glycans is a novel feature in dipteran glycomes; these structures have the potential to act as additional anionic glycan ligands involved in parasite interactions with the vector host.
Palavras-Chave: Glycans; Glycomics; HPLC; Insects; Mass spectrometric; Oligosaccharides
Imprenta: Journal of Proteomics, v. 126, p. 172-188, 2015
Identificador do objeto digital: 10.1016/j.jprot.2015.05.030
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Dengue
Data de publicação: 2015
