Autor(es): Becnel James J,Pridgeon Julia W

Resumo: Mosquito iridescent viruses (MIV) are members of the genus Chloriridovirus that currently contains only the type IIV-3 from Aedestaeniorhynchus. The complete genome of invertebrate iridescent virus -3 (IIV-3) has been sequenced and the availability of a tissue culture system would facilitate functional genomic studies. This investigation, using quantitative PCR and electron microscopy, has determined that the mosquito cell lines Aedes aegypti (Aag2), Aedes albopictus (C6/36) and Anopheles gambiae (4a3A) as well as the lepidopteran cell line from Spodoptera frugiperda (SF9) are permissive to IIV-3 infection. However, IIV-3 infection remained longer in Aag2 and C6/36 cells. Virus produced in C6/36 cell line was infectious to larvae of A. taeniorhynchus by injection and per os. Ultrastructural examination of 4a3A and SF9 cells infected with IIV-3 revealed an unusual feature, where virions were localized to mitochondria. It is speculated that containment with mitochondria may play a role in the lack of persistence in these cell lines.

Palavras-Chave: Aedes taeniorhynchus; Chloriridovirus; Mosquito iridescent virus; Mosquito; Tissue culture; Ultrastructure

Imprenta: Journal of Invertebrate Pathology, v. 108, n. 1, p. 40-45, 2011

Identificador do objeto digital: 10.1016/j.jip.2011.06.008

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Genome ; Aedes aegypti - Pathogenesis ; Aedes aegypti - virus

Data de publicação: 2011