Autor(es): Yang Y J,Davies D M

Resumo: Trypsin and chymotrypsin occurred in the midgut of all stages of Aedes aegypti. In larvae and pharate pupae, both enzymes were very active, whereas only trypsin was highly active in adult females after a blood meal. the low activity of trypsin and chymotrypsin between the larval-pupal and pupal-adult ecdyses presumably relates to the lack of feeding during this period. Chymotrypsins from the larva and adult were simultaneously eluted through Sephadex G-100, and most of the enzyme was positively charged on acrylamide-gel electrophoresis at pH 8·6, 7·0, and 5·5. Mosquito chymotrypsin was inhibited by human and horse ?-globulins, and the former was revealed as a competitive inhibitor. Diphenylcarbamyl chloride inactivated mosquito chymotrypsin both in vivo and in vitro. The value of GPNA for mosquito chymotrypsin assay was demonstrated.

Imprenta: Journal of Insect Physiology, v. 17, n. 1, p. 117-131, 1971

Identificador do objeto digital: 10.1016/0022-1910(71)90048-5

Descritores: Aedes aegypti - Proteins

Data de publicação: 1971

INDICADORES

ACESSO