Autor(es): Sohal R S,Copeland E

Resumo: The larvae of Aedes aegypti (L.) absorb water and inorganic ions from a hypotonic environment through their anal papillae. The ultrastructure of the epithelial cells of the anal papillae of A. aegypti was studied in larvae raised in different salinities. The larvae were reared in NaCl solutions having salinities of 0·039%, 0·67%, and 1·02% NaCl. The anal papillae of fourth-instar larvae were fixed in buffered glutaraldehyde followed by buffered osmium tetroxide, and embedded in Epon 812 for electron microscope studies. In the larvae raised in hypotonic solution of 0·039% NaCl, the plasma membrane on the cuticular surface of the epithelial cells is arranged into folds. The mitochondria lie in close association with these folds. The plasma membrane on the basal surface of the cells invaginates to form an anastomosing network. In the larvae raised in an approximately isotonic solution containing 0·67% NaCl, and in the hypertonic solution containing 1·02% NaCl, the surface of the plasma membrane on the cuticular surface of the epithelial cells is comparatively reduced as compared with that of the animals raised in the hypotonic solution. Fewer mitochondria lie between the folds of the plasma membrane. This study suggests that the intake of the inorganic ions through the anal papillae may be regulated by variations in the surface area of the plasma membrane on the cuticular surface of the epithelial cells.

Imprenta: Journal of Insect Physiology, v. 12, n. 4, p. 429-434, 1966

Identificador do objeto digital: 10.1016/0022-1910(66)90006-0

Descritores: Aedes aegypti - Cell

Data de publicação: 1966

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