Autor(es): Pham D Q-D,Douglass P L,Chavez C A,Shaffer J J

Resumo: In the yellow fever mosquito Aedes aegypti, the ferritin heavy-chain homologue (HCH) gene is induced by blood feeding. This suggests that ferritin may serve as a cytotoxic protector against the oxidative challenge of the blood meal and may be essential for the survival of the insect. In this study, various cis-acting elements for the gene were identified and mapped. Transfection assays showed that the strength and activity of a subset of these elements are orientation-dependent. The shift observed for the ferritin HCH cis-acting elements is unique among known ferritin genes. DNase I footprinting data together with Transfac analyses identified a number of putative sites known for their involvement in developmental and cell proliferation processes.

Palavras-Chave: Mosquito ferritin; Heavy-chain homologue; Gene regulation; Orientation-independent; Iron

Imprenta: Insect Molecular Biology, v. 14, n. 3, p. 223-236, 2005

Identificador do objeto digital: 10.1111/j.1365-2583.2004.00550.x

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins

Data de publicação: 2005