Autor(es): Lu S J,Pennington J E,Stonehouse A R,Mobula M M,Wells M A

Resumo: In the female mosquito Aedes aegypti, trypsin expression is largely biphasic. Early trypsin synthesis, which is regulated at the translational level relative to feeding, peaks in the first few hours post-blood meal. Late trypsin expression is regulated at the transcriptional level, and peaks 18-24h post-blood meal. It was proposed that early trypsin activity released unknown factors during digestion of a meal that caused activation of transcription of the late trypsin gene. This connection between early trypsin activity and late trypsin expression was dependent on the fact that feeding a single trypsin inhibitor, soybean trypsin inhibitor (STI), which blocked early trypsin activity, also blocked late trypsin expression. We show in this study that feeding different trypsin inhibitors which effectively blocked early trypsin activity did not result in reduced late trypsin expression. We also found that a different lot of STI failed to cause inhibition of late trypsin transcription, although it was effective in inhibiting early trypsin activity. In addition, using RNAi methodology to reduce the level of early trypsin expression had no effect on the level of late trypsin expression. We conclude that early trypsin activity is not necessary for the transcriptional activation of late trypsin and that the previous results were due to the effect of a cytotoxic agent present in some, but not all preparations of STI.

Palavras-Chave: RNAi; Trypsin inhibitor; Regulation; Transcription

Imprenta: Insect Biochemistry and Molecular Biology, v. 36, n. 4, p. 336-343, 2006

Identificador do objeto digital: 10.1016/j.ibmb.2006.01.011

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Public health

Data de publicação: 2006

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