Structural organization of the mitochondrial DNA control region in Aedes aegypti
Autor(es): Dueñas Juan C Rondan,Gardenal Cristina N,Llinás Guillermo Albrieu,Panzetta-Dutari Graciela M
Resumo: The complete A+T - rich region of Aedes aegypti mitochondrial DNA has been cloned and sequenced. In Argentinean populations of the species, a polymorphism in the length of the amplified fragment was observed. Nucleotide sequence comparison of the shortest and longest A+T - rich amplified fragments detected revealed the presence of 2 types of tandemly repeated blocks. The size variation observed in natural populations is mainly due to the presence of a variable number of a 181 bp tandem repeat unit, located toward the 12S rRNA gene end. The size of the longest A+T - rich region was of 2070 bp, representing the largest control sequence reported for any mosquito species. Few relevant short blocks of primary-sequence similarity conserved in the control region of mosquitoes and other insects were detected scattered throughout the whole region. Five putative stem-loop secondary structures were found, one of them flanked by conserved sequences described in other insects. Our results suggest that there are no universal models of structure-function relations in the control region of insect mtDNA. In addition, we identified a short A+T - rich variable segment in the Ae. aegyti control region that would be suitable for population genetic studies.
Palavras-Chave: Mitocondrial DNA; A+T - rich region; Repeated elements; Conserved blocks; Aedes aegypti
Imprenta: Genome / National Research Council Canada, v. 49, n. 8, p. 931-937, 2006
Identificador do objeto digital: 10.1139/g06-053
Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis
Data de publicação: 2006
