Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development
Autor(es): Haugen Morgan,Tomchaney Michael,Kast Kristopher,Flannery Ellen,Clemons Anthony,Jacowski Caitlin,Simanton Holland Wendy,Le Christy,Severson David,Duman-Scheel Molly
Resumo: Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol for whole-mount in situ hybridization can be used to analyze gene expression in Ae. aegypti embryos and larvae, a critical aspect of understanding developmental gene function in this vector mosquito.
Imprenta: Cold Spring Harbor Protocols, v. 2010, n. 10, p. pdb.prot5509, 2010
Identificador do objeto digital: 10.1101/pdb.prot5509
Descritores: Aedes aegypti - Genome ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Molecular methods ; Aedes aegypti - Dengue ; Aedes aegypti - Epidemic ; Aedes aegypti - Public health
Data de publicação: 2010
