Autor(es): Grard Gilda, Caron Mélanie, Mombo Illich Manfred, Nkoghe Dieudonné, Mboui Ondo Statiana, Jiolle Davy, Fontenille Didier, Paupy Christophe, Leroy Eric Maurice

Resumo: Chikungunya - dengue viruses emerged in Gabon in 2007, with large outbreaks primarily affecting the capital Libreville - several northern towns. Both viruses subsequently spread to the south-east of the country, with new outbreaks occurring in 2010. The mosquito species Aedes albopictus, that was known as a secondary vector for both viruses, recently invaded the country - was the primary vector involved in the Gabonese outbreaks. We conducted a retrospective study of human sera - mosquitoes collected in Gabon from 2007 to 2010, in order to identify other circulating arboviruses. Sample collections, including 4312 sera from patients presenting with painful febrile disease, - 4665 mosquitoes belonging to 9 species, split into 247 pools (including 137 pools of Aedes albopictus), were screened with molecular biology methods. Five human sera - two Aedes albopictus pools, all sampled in an urban setting during the 2007 outbreak, were positive for the flavivirus Zika (ZIKV). The ratio of Aedes albopictus pools positive for ZIKV was similar to that positive for dengue virus during the concomitant dengue outbreak suggesting similar mosquito infection rates -, presumably, underlying a human ZIKV outbreak. ZIKV sequences from the envelope - NS3 genes were amplified from a human serum sample. Phylogenetic analysis placed the Gabonese ZIKV at a basal position in the African lineage, pointing to ancestral genetic diversification - spread. We provide the first direct evidence of human ZIKV infections in Gabon, - its first occurrence in the Asian tiger mosquito, Aedes albopictus. These data reveal an unusual natural life cycle for this virus, occurring in an urban environment, - potentially representing a new emerging threat due to this novel association with a highly invasive vector whose geographic range is still exp-ing across the globe.

Imprenta: PLoS Neglected Tropical Diseases, v. 8, n. 2, p. e2681, 2014

Identificador do objeto digital: 10.1371/journal.pntd.0002681

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health

Data de publicação: 2014

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