Vectorial capacity of Culex gelidus (Theobald) mosquitoes to certain viruses of public health importance in India
Autor(es): Sudeep A B, Ghodke Y S, George R P, Ingale V S, Dhaigude S D, Gokhale M D
Resumo: Culex gelidus, a widely prevalent mosquito in India - Southeast Asia region, is an important vector of Japanese encephalitis virus (JEV). Experimental studies have shown its potential to transmit West Nile, Kunjin, Murray Valley encephalitis - Ross River viruses. An attempt was therefore made to study its susceptibility - vector competence to some of the arboviruses of public health importance in India. Mosquitoes were infected with six viruses, viz. JEV, chikungunya (CHIKV), Ch-ipura (CHPV), Chittoor (CHITV), Ingwavuma (INGV) - Umbre (UMBV) by intra thoracic inoculation to determine virus susceptibility - vector competence. Growth kinetics of the viruses were studied by determining the titres of inoculated mosquitoes on different days post-infection by titration in Vero E6 cells. Vector competence was studied by detecting the presence of the viruses in saliva of infected mosquitoes. All the six viruses were replicated in Cx. gelidus. JEV, CHPV, CHIKV - CHITV yielded > 5 log10TCID50/ml virus while UMBV - INGV yielded approx 4log10TCID50/ml virus. JEV, CHIKV - CHITV could be detected in the saliva of the infected mosquitoes, while CHPV, INGV - UMBV could not be detected in the saliva of the infected mosquitoes. Replication potential - vector competence of Cx. gelidus to some of the viruses of public health importance in India, viz. JEV, CHIKV, CHITV etc, pose a serious threat to general population, especially in the wake of spurt in its population in certain parts of India.
Palavras-Chave: Chikungunya virus; Chittoor virus; Culex gelidus; Replication; Japanese encephalitis virus; Vector competence
Imprenta: Journal of Vector Borne Diseases, v. 52, n. 2, p. 153-158, 2015
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya Virus - Virus ; Chikungunya virus - Public health
Data de publicação: 2015
