Utilization of an eilat virus-based chimera for serological detection of chikungunya infection
Autor(es): Erasmus Jesse H, Needham James, Raychaudhuri Syamal, Diamond Michael S, Beasley David W C, Morkowski Stan, Salje Henrik, Fernandez Salas Ildefonso, Kim Dal Young, Frolov Ilya, Nasar Farooq, Weaver Scott C
Resumo: In December of 2013, chikungunya virus (CHIKV), an alphavirus in the family Togaviridae, was introduced to the isl- of Saint Martin in the Caribbean, resulting in the first autochthonous cases reported in the Americas. As of January 2015, local - imported CHIKV has been reported in 50 American countries with over 1.1 million suspected cases. CHIKV causes a severe arthralgic disease for which there are no approved vaccines or therapeutics. Furthermore, the lack of a commercially available, sensitive, - affordable diagnostic assay limits surveillance - control efforts. To address this issue, we utilized an insect-specific alphavirus, Eilat virus (EILV), to develop a diagnostic antigen that does not require biosafety containment facilities to produce. We demonstrated that EILV/CHIKV replicates to high titers in insect cells - can be applied directly in enzyme-linked immunosorbent assays without inactivation, resulting in highly sensitive detection of recent - past CHIKV infection, - outperforming traditional antigen preparations.
Imprenta: PLoS Neglected Tropical Diseases, v. 9, n. 10, p. e0004119, 2015
Identificador do objeto digital: 10.1371/journal.pntd.0004119
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2015
