The use of serum spotted onto filter paper for diagnosing and monitoring Chikungunya virus infection
Autor(es): Matheus Séverine, Huc Patricia, Labeau Bhety, Bremand Laetitia, Enfissi Antoine, Merle Olivier, Flusin Olivier, Rousset Dominique, Leparc-Goffart Isabelle
Resumo: The recent emergence of Chikungunya Virus (CHIKV) in the Americas constitutes a major public health problem on this continent, where the mosquito vector is widespread. The rapid diagnosis of suspected cases is essential for the monitoring - control of this ongoing outbreak. However, this requires reliable tools that are difficult to establish in areas without specialized laboratories. The aim was to evaluate the performances of serum samples spotted onto filter paper for molecular - serological diagnosis of Chikungunya infection. Analyses were performed from frozen sera - serum spotted onto filter paper provided from 121 Chikungunya suspected cases collected at a biological laboratory on Saint-Martin Isl-. This approach performed well in comparisons with st-ard methods, with a sensitivity of 100% - a specificity of 93.6% for the combined technical approaches (RT-PCR - serological results). Comparisons of serum samples spotted onto filter paper - frozen samples showed a concordance rate of 94.8% in molecular tests - 98.2% in serological tests. This simple sampling technique could overcome the problems of the lack of efficient CHIKV diagnosis tools in remote regions, providing good results regardless of the molecular or serological approach used. This simple filter paper-based method can be used to diagnose both chikungunya - dengue infections, as previously demonstrated following transport at ambient temperature to specialized laboratories. Given the set-up costs - high performance of this method, it could be recommended for the monitoring - control of Chikungunya virus expansion in the Americas - in other affected regions.
Palavras-Chave: Chikungunya; Diagnosis; Filter paper
Imprenta: Journal of Clinical Virology, v. 71, p. 89-92, 2015
Identificador do objeto digital: 10.1016/j.jcv.2015.08.008
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Molecular methods ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serological diagnosis ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2015
