Autor(es): Mousson Laurence, Zouache Karima, Arias-Goeta Camilo, Raquin Vincent, Mavingui Patrick, Failloux Anna-Bella

Resumo: The chikungunya (CHIK) outbreak that struck La Reunion Isl- in 2005 was preceded by few human cases of Dengue (DEN), but which surprisingly did not lead to an epidemic as might have been expected in a non-immune population. Both arboviral diseases are transmitted to humans by two main mosquito species, Aedes aegypti - Aedes albopictus. In the absence of the former, Ae. albopictus was the only species responsible for viral transmission on La Reunion Isl-. This mosquito is naturally super-infected with two Wolbachia strains, wAlbA - wAlbB. While Wolbachia does not affect replication of CHIK virus (CHIKV) in Ae. albopictus, a similar effect was not observed with DEN virus (DENV). To underst- the weak vectorial status of Ae. albopictus towards DENV, we used experimental oral infections of mosquitoes from La Reunion Isl- to characterize the impact of Wolbachia on DENV infection. Viral loads - Wolbachia densities were measured by quantitative PCR in different organs of Ae. albopictus where DENV replication takes place after ingestion. We found that: (i) Wolbachia does not affect viral replication, (ii) Wolbachia restricts viral density in salivary gl-s, - (iii) Wolbachia limits transmission of DENV, as infectious viral particles were only detected in the saliva of Wolbachia-uninfected Ae. albopictus, 14 days after the infectious blood-meal. We show that Wolbachia does not affect the replication of DENV in Ae. albopictus. However, Wolbachia is able to reduce viral infection of salivary gl-s - limit transmission, suggesting a role of Wolbachia in naturally restricting the transmission of DENV in Ae. albopictus from La Reunion Isl-. The extension of this conclusion to other Ae. albopictus populations should be investigated.

Imprenta: PLoS Neglected Tropical Diseases, v. 6, n. 12, p. e1989, 2012

Identificador do objeto digital: 10.1371/journal.pntd.0001989

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - Real Time PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health

Data de publicação: 2012

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