Autor(es): Haac Mary Etna, Anderson Michelle A E, Eggleston Heather, Myles Kevin M, Adelman Zach N

Resumo: Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses - chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation - protection from transposable elements. Double-str-ed RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA - exo-siRNA pathway is aided by the dsRBPs Loquacious (Loqs-PB, Loqs-PD) - R2D2, respectively. However, this functional specialization has not been investigated in other dipterans. We were unable to detect Loqs-PD in Ae. aegypti; analysis of other dipteran genomes demonstrated that this isoform is not conserved outside of Drosophila. Overexpression experiments - small RNA sequencing following depletion of each dsRBP revealed that R2D2 - Loqs-PA cooperate non-redundantly in siRNA production, - that these proteins exhibit an inhibitory effect on miRNA levels. Conversely, Loqs-PB alone interacted with mosquito dicer-1 - was essential for full miRNA production. Mosquito Loqs interacted with both argonaute 1 - 2 in a manner independent of its interactions with dicer. We conclude that the functional specialization of Loqs-PD in Drosophila is a recently derived trait, - that in other dipterans, including the medically important mosquitoes, Loqs-PA participates in both the miRNA - endo-siRNA based pathways.

Imprenta: Nucleic Acids Research, v. 43, n. 7, p. 3688-3700, 2015

Identificador do Objeto Digital: 10.1093/nar/gkv152

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue

Data de Publicação: 2015