The clinical, serological and molecular diagnosis of emerging dengue infection at a tertiary care institute in southern, India
Autor(es): Neeraja Mamidi, Lakshmi Vemu, Dash P K, Parida M M, Rao P V L
Resumo: Dengue is an acute viral infection which presents as uneventful pyrexia to a fatal complication. This infection is increasingly being recognized as the world's major emerging tropical disease - an important public health problem. This article highlights the clinical manifestations of Dengue virus infection - the various molecular tests that were used for its laboratory diagnosis. Serum samples from 713 suspected cases of Dengue were collected between August - December 2007. The clinical profiles of 123 hospitalized patients were analyzed. Serology, RT- PCR, virus isolation - sequencing were done. The most common clinical symptoms were fever, thrombocytopenia, rash - elevated liver enzymes. The demonstration of the Dengue RNA in 5.16% samples, the detection of Dengue specific IgM antibodies in 18% samples - the isolation of the DENV-4 - the DENV-3 viruses from the clinical samples confirmed this Dengue outbreak. A co -infection with Chikungunya was observed in 2.06% of the cases. The phylogenetic analysis revealed that the Indian Dengue-4 isolates from this outbreak belonged to the genotype I. This study clearly indicated the sudden dominance of DENV-4 in an Indian Dengue outbreak. The surveillance of the Dengue viruses needs to be closely monitored for the emergence of newer serotype(s) in hitherto unknown areas.
Palavras-Chave: Chikungunya; Dengue fever; Dengue-3; Dengue-4; Phylogenetic analysis
Imprenta: Journal of Clinical and Diagnostic Research, v. 7, n. 3, p. 457-461, 2013
Identificador do objeto digital: 10.7860/JCDR/2013/4786.2798
Descritores: Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Molecular methods ; Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serological diagnosis ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2013
