The acute phase of Chikungunya virus infection in humans is associated with strong innate immunity and T CD8 cell activation
Autor(es): Wauquier Nadia, Becquart Pierre, Nkoghe Dieudonné, Padilla Cindy, Ndjoyi-Mbiguino Angélique, Leroy Eric M
Resumo: Rapidly spreading to new regions, including the isl-s of the Indian Ocean, Central Africa, - Europe, Chikungunya fever is becoming a major problem of public health. Unlike other members of the alphavirus genus, immune responses to Chikungunya virus (CHIKV) have been poorly investigated. We conducted a large ex vivo multiplex study of 50 cytokine, chemokine, - growth factor plasma profiles in 69 acutely infected patients from the Gabonese outbreak of 2007. We also assessed a phenotypic study of T lymphocyte responses during human acute CHIKV infection. CHIKV infection in humans elicited strong innate immunity involving the production of numerous proinflammatory mediators. Interestingly, high levels of Interferon (IFN) ? were consistently found. Production of interleukin (IL) 4, IL-10, - IFN-? suggested the engagement of the adaptive immunity. This was confirmed by flow cytometry of circulating T lymphocytes that showed a CD8+ T lymphocyte response in the early stages of the disease, - a CD4+ T lymphocyte mediated response in the later stages. For the first time to our knowledge, we found evidence of CD95-mediated apoptosis of CD4+ T lymphocytes during the first 2 days after symptoms onset, ex vivo. Together, our findings suggest that strong innate immunity is required to control CHIKV infection.
Imprenta: The Journal of Infectious Diseases, v. 204, n. 1, p. 115-123, 2011
Identificador do objeto digital: 10.1093/infdis/jiq006
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - T lymphocytes ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2011
