Subunit vaccine formulations based on recombinant envelope proteins of Chikungunya virus elicit balanced Th1/Th2 response and virus-neutralizing antibodies in mice
Autor(es): Khan Mohsin, Dhanwani Rekha, Rao Putcha Venkata Lakshamana, Parida Manmohan
Resumo: The recent resurgence of Chikungunya virus in India - Indian Ocean Isl-s with unusual clinical severity is a matter of great public health concern. Despite the fact that CHIKV resurgence is associated with epidemic of unprecedented magnitude, none of the vaccine c-idate has been approved so far. The envelope protein E1 - E2 being the major immunodominant structural proteins with crucial role in virus attachment - entry, can prove to be potential vaccine c-idates. In the present study, the immunogenic potential of bacterially expressed CHIKE1 - CHIKE2 recombinant proteins along with various adjuvants is reported. Assessment of the protective efficacy of both the vaccine formulations was further confirmed by both in vitro - in vivo neutralisation tests. Splenocytes from immunized mice, cultured in vitro when stimulated with the vaccine antigens revealed induction of very high levels of both pro- - anti- inflammatory cytokines indicating a balance of Th1 - Th2 response.
Palavras-Chave: Chikungunya vaccine; Envelope protein; Humoral response; Cell mediated response; Cytokine profiling
Imprenta: Virus Research, v. 167, n. 2, p. 236-246, 2012
Identificador do objeto digital: 10.1016/j.virusres.2012.05.004
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Cytokines ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2012
