Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains
Autor(es): Maassen C B, van Holten-Neelen C, Balk F, den Bak-Glashouwer M J, Leer R J, Laman J D, Boersma W J, Claassen E
Resumo: Different Lactobacillus strains are frequently used in consumer food products. In addition, recombinant lactobacilli which contain novel expression vectors can now be used in immunotherapeutic applications such as oral vaccination strategies - in T cell tolerance induction approaches for autoimmune disease. Both for food - clinical applications of lactobacilli, proper selection of wild type strains is crucial. For that purpose, eight different common Lactobacillus strains were analysed with respect to mucosal induction of pro- - anti-inflammatory cytokines, IgA-producing plasma cells in the gut, as well as systemic antibody responses against a parenterally administered antigen. Immunohistochemical analysis of cytokine-producing cells in the gut villi showed no significant induction of the cytokines IL-1alpha, IFN-gamma, IL-4 or IL-10 after oral administration of wild type Lactobacillus strains. In contrast, oral administration of L. reuteri - L. brevis induced expression of the proinflammatory/Th1 cytokines TNF-alpha, IL-2 -/or IL-1beta. Oral administration of these two strains - L. fermentum also significantly enhanced the IgG response against parenterally administered haptenated chicken gamma globulin (TNP-CGG). The five other strains did not show this adjuvanticity. L. reuteri induced relatively high levels of IgG2a compared to L. murines, a nonadjuving Lactobacillus strain. These findings imply that different Lactobacillus strains induce distinct mucosal cytokine profiles - possess differential intrinsic adjuvanticity. This suggests that rational Lactobacillus strain selection provides a strategy to influence cytokine expression - thereby influence immune responses.
Palavras-Chave: Probiotic; Mucosal immune response; Immunohistochemistry
Imprenta: Vaccine, v. 18, n. 23, p. 2613-2623, 2000
Identificador do objeto digital: 10.1016/S0264-410X(99)00378-3
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2000
