Standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors Aedes aegypti and Aedes albopictus (Diptera: Culicidae) and II and Egg storage and hatching
Autor(es): Zheng Min-Lin, Zhang Dong-Jing, Damiens David D, Lees Rosemary Susan, Gilles Jeremie R L
Resumo: Management of large quantities of eggs will be a crucial aspect of the efficient - sustainable mass production of mosquitoes for programmes with a Sterile Insect Technique component. The efficiency of different hatching media - effectiveness of long term storage methods are presented here. The effect on hatch rate of storage duration - three hatching media was analysed: deionized water, boiled deionized water - a bacterial broth, using Two-way ANOVA - Post hoc Tukey tests, - the Pearson correlation coefficient was used to find the effect on the proportion of collapsed eggs. Two long term storage methods were also tested: conventional storage (egg paper strips stored in zip lock bags within a sealed plastic box), - water storage (egg papers in a covered plastic cup with deionized water). Regression analyses were used to find the effect of water storage - storage duration on hatch rate. Both species hatched most efficiently in bacterial broth. Few eggs hatched in deionized water, - pre-boiling the water increased the hatch rate of Ae. aegypti, but not Ae. albopictus. A hatch rate greater than 80% was obtained after 10 weeks of conventional storage in Ae. aegypti - 11 weeks in Ae. albopictus. After this period, hatching decreased dramatically; no eggs hatched after 24 weeks. Storing eggs in water produced an 85% hatch rate after 5 months in both species. A small but significant proportion of eggs hatched in the water, probably due to combined effects of natural deoxygenation of the water over time - the natural instalment hatching typical of the species. The demonstrated efficiency of the bacterial broth hatching medium for both Ae. albopictus - Ae. aegypti facilitates mass production of these two important vector species in the same facility, with use of a common hatching medium reducing cost - operational complexity. Similarly the increased hatch rate of eggs stored in water would allow greater flexibility of egg management in a large programme over the medium term, particularly if oxygenation of the water by bubbling oxygen through the storage tray could be applied to prevent hatching during storage.
Palavras-Chave: Bacterial broth; Boiled water; Hatch rate; Egg storage; Mass rearing; Mosquito production; Aedes; Dengue; Chikungunya
Imprenta: Parasites & Vectors, v. 8, p. 348, 2015
Identificador do objeto digital: 10.1186/s13071-015-0951-x
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2015
