Spatio-temporal distribution of Aedes aegypti (Diptera: Culicidae) mitochondrial lineages in cities with distinct dengue incidence rates suggests complex population dynamics of the dengue vector in Colombia
Autor(es): Jaimes-Dueñez Jeiczon, Arboleda Sair, Triana-Chávez Omar, Gómez-Palacio Andrés
Resumo: Aedes aegypti is the primary vector of the four serotypes of dengue virus (DENV1-4), Chikungunya - yellow fever virus to humans. Previous population genetic studies have revealed a particular genetic structure among the vector populations in the Americas that suggests differences in the ability to transmit DENV. In Colombia, despite its high epidemiologic importance, the genetic population structure - the phylogeographic depiction of Ae. aegypti, as well as its relationship with the epidemiologic l-scapes in cities with heterogeneous incidence levels, remains unknown. We conducted a spatiotemporal analysis with the aim of determining the genetic structure - phylogeography of Colombian populations of Ae. aegypti among cities with different eco-epidemiologic characteristics with regard to DENV. Mitochondrial cytochrome oxidase C subunit 1 (COI)--NADH dehydrogenase subunit 4 (ND4) genes were sequenced - analyzed from 341 adult mosquitoes collected during 2012 - 2013 in the Colombian cities of Bello, Riohacha - Villavicencio, which exhibit low, medium - high levels of incidence of DENV, respectively. The results demonstrated a low genetic differentiation over time - a high genetic structure between the cities due to changes in the frequency of two highly supported genetic groups. The phylogeographic analyses indicated that one group (associated with West African populations) was found in all the cities throughout the sampling while the second group (associated with East African populations) was found in all the samples from Bello - in only one sampling from Riohacha. Environmental factors such as the use of chemical insecticides showed a significant correlation with decreasing genetic diversity, indicating that environmental factors affect the population structure of Ae. aegypti across time - space in these cities. Our results suggest that two Ae. aegypti lineages are present in Colombia; one that is widespread - related to a West African conspecific, - a second that may have been recently introduced - is related to an East African conspecific. The first lineage can be found in cities showing a high incidence of dengue fever - the use of chemical insecticides, whereas the second is present in cities showing a low incidence of dengue fever where the use of chemical insecticides is not constant. This study helps to improve our knowledge of the population structure of Ae. aegypti involved in the diversity of dengue fever epidemiology in Colombia.
Imprenta: PLoS Neglected Tropical Diseases, v. 9, n. 4, p. e0003553, 2015
Identificador do objeto digital: 10.1371/journal.pntd.0003553
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - DNA ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health
Data de publicação: 2015
