Autor(es): Kam Yiu-Wing, Pok Kwoon-Yong, Eng Kai Er, Tan Li-Kiang, Kaur Simrandeep, Lee Wendy W L, Leo Yee-Sin, Ng Lee-Ching, Ng Lisa F P

Resumo: Chikungunya virus (CHIKV) - clinically-related arboviruses cause large epidemics with serious economic - social impact. As clinical symptoms of CHIKV infections are similar to several flavivirus infections, good detection methods to identify CHIKV infection are desired for improved treatment - clinical management. The strength of anti-E2EP3 antibody responses was explored in a longitudinal study on 38 CHIKV-infected patients. We compared their anti-E2EP3 responses with those of patients infected with non-CHIKV alphaviruses, or flaviviruses. E2EP3 cross-reactive samples from patients infected with non-CHIKV viruses were further analyzed with an in vitro CHIKV neutralization assay. CHIKV-specific anti-E2EP3 antibody responses were detected in 72% to 100% of patients. Serum samples from patients infected with other non-CHIKV alphaviruses were cross-reactive to E2EP3. Interestingly, some of these antibodies demonstrated clearly in vitro CHIKV neutralizing activity. Contrastingly, serum samples from flaviviruses-infected patients showed a low level of cross-reactivity against E2EP3. Using CHIKV E2EP3 as a serology marker not only allows early detection of CHIKV specific antibodies, but would also allow the differentiation between CHIKV infections - flavivirus infections with 93% accuracy, thereby allowing precise acute febrile diagnosis - improving clinical management in regions newly suffering from CHIKV outbreaks including the Americas.

Imprenta: PLoS Neglected Tropical Diseases, v. 9, n. 1, p. e3445, 2015

Identificador do objeto digital: 10.1371/journal.pntd.0003445

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biochemistry ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2015

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