Autor(es): Yathi Krishna Kammara, Joseph Julia Mary, Bhasker Salini, Kumar Ramesh, Chinnamma Mohankumar

Resumo: Chikungunya is an acute febrile illness caused by an alpha virus technically called as CHIK virus. A smaller size of CHIK virus E1 coat protein -11 kDa was expressed in prokaryotic expression system. The recombinant protein was purified - confirmed by western blot analysis. The positions of the antigenic domain in the protein were identified - the immunoreactivity of recombinant protein with anti-CHIK IgM antibodies was ascertained. The antigen showed an 88% sensitivity - 100% specificity by Indirect ELISA. No cross reactivity of the antigen was observed with anti-Dengue virus serum samples. The results strongly support that the recombinant CHIK coat protein could be used as a diagnostic antigen for the detection of Chikungunya by Indirect ELISA. The relevance of a smaller size recombinant antigen highlights its large scale application in serodiagnosis of CHIK virus since bacterial expression is more simple - cost effective than eukaryotic system.

Palavras-Chave: Chikungunya; E1 coat protein; Recombinant antigen; Prokaryotic expression; Specificity; ELISA

Imprenta: Journal of Immunological Methods, v. 372, n. 1-2, p. 171-176, 2011

Identificador do objeto digital: 10.1016/j.jim.2011.07.011

Descritores: Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2011

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