Real-time whole-body visualization of Chikungunya virus infection and host interferon response in zebrafish
Autor(es): Palha Nuno, Guivel-Benhassine Florence, Briolat Valérie, Lutfalla Georges, Sourisseau Marion, Ellett Felix, Wang Chieh-Huei, Lieschke Graham J, Herbomel Philippe, Schwartz Olivier, Levraud Jean-Pierre
Resumo: Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of ?14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils - hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis - host responses to a human virus.
Imprenta: PLoS Pathogens, v. 9, n. 9, p. e1003619, 2013
Identificador do objeto digital: 10.1371/journal.ppat.1003619
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Cytopathology ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Public health
Data de publicação: 2013
