Rapid identification of Chikungunya and Dengue virus by a real-time reverse transcription-loop-mediated isothermal amplification method
Autor(es): Lu Xi, Li Xiaobo, Mo Ziyao, Jin Faguang, Wang Boliang, Zhao Hongbo, Shan Xiaoxiao, Shi Lei
Resumo: Both Chikungunya - Dengue virus belong to the acute arthropod-borne viruses. Because of the lack of specific symptoms, it is difficult to distinguish the two infections based on clinical manifestations. To identify - quantitatively detect Chikungunya - Dengue viruses, a real-time accelerated reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) platform was developed, - 26-confirmed RNA samples, 42 suspects, - 18 healthy serum samples were evaluated by the method. The RT-polymerase chain reaction (PCR) - cDNA sequencing were used as references. The results showed that it could identify the Chikungunya - Dengue virus RNA correctly in all antibody-positive samples within 1 hour, without any cross-reactions. The virus load of the positive samples was quantitatively detected with a turbidimeter. The sensitivity was 100% - specificity was 95.25%. The findings indicate that the RT-LAMP is an effective method for rapid quantity detection of Chikungunya virus - Dengue virus in serum samples with convenient operation, high specificity, - high sensitivity.
Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 87, n. 5, p. 947-953, 2012
Identificador do objeto digital: 10.4269/ajtmh.2012.11-0721
Descritores: Chikungunya virus - DNA ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Molecular structure ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue
Data de publicação: 2012
