Rapid detection of Chikungunya virus in laboratory infected Aedes aegypti by Reverse-Transcriptase- Polymerase Chain Reaction (RT-PCR)
Autor(es): Rohani A, Yulfi H, Zamree I, Lee H L
Resumo: A study of chikungunya virus was carried out to establish Reverse Transcriptase- Polymerase Chain Reaction (RT-PCR) as a rapid detection technique of the virus. The susceptibility of lab-colonized Aedes aegypti to chikungunya virus was also determined. Artificial membrane feeding technique was used to orally feed the mosquitoes with a human isolate of chikungunya virus. A total of 100 fully engorged female Ae. aegypti were obtained - maintained for 7 days. Seventy of them survived - then pooled at 10 individuals per pool. Total RNA was extracted from the samples - RT-PCR amplifications were carried out. Five out of 7 pools showed positive PCR b- at 350-bp, indicating Ae. aegypti is a potential vector of chikungunya virus. The minimum infection rate (MIR) was 71% within these laboratory colonies. RT-PCR is a sensitive technique that is useful in detecting infected mosquitoes in epidemic areas. This technique can de used as a rapid detection method - provide an early virologic surveillance systems of chikungunya virus infected mosquitoes.
Imprenta: Tropical Biomedicine, v. 22, n. 2, p. 149-154, 2005
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - PCR detection ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2005
