Rapid and real-time detection technologies for emerging viruses of biomedical importance
Autor(es): Parida M M
Resumo: The development of technologies with rapid - sensitive detection capabilities - increased throughput have become crucial for responding to greater number threats posed by emerging - re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, -/or detection of antibodies,which are not very sensitive - specific. The recent advances in molecular biology techniques in the field of genomics - proteomics greatly facilitate the rapid identification with more accuracy. We have developed two real-time assays ie., SYBR green I based real time reverse transcription polymerase chain reaction (RT-PCR) - RT-loop-mediated isothermal amplification (LAMP) assay for rapid detection as well as typing of some of the emerging viruses of biomedical importance viz. dengue, Japanese encephalitis, chikungunya, west Nile, severe acute respiratory syndrome virus (SARS) etc. Both these techniques are capable of detection - differentiation as well as quantifying viral load with higher sensitivity, rapidity, specificity. One of the most important advantages of LAMP is its field applicability, without requirement of any sophisticated equipments. Both these assays have been extensively evaluated - validated with clinical samples of recent epidemics from different parts of India. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision - accuracy in future.
Palavras-Chave: Emerging viruses; LAMP; Rapid detection; Real-time PCR
Imprenta: Journal of Biosciences, v. 33, n. 4, p. 617-628, 2008
Descritores: Chikungunya virus - DNA ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2008
