Radial haemolysis in gel: A comparative study with Chikungunya, Japanese encephalitis and rubella viruses, representatives of three genera of Togaviridae family
Autor(es): Gaidamovich S Y, Melnikova E E, Väänänen P, Penttinen K
Resumo: Optimal conditions of radial haemolysis in gel (HIG) with Chikungunya (Chik) - Japanese encephalitis (JE) viruses were developed - compared with those of rubella virus. Sheep erythrocytes were found preferable for HIG with Chik - JE viruses, unlike rubella virus, where goose erythrocytes gave best results. Rooster erythrocytes were completely negative in the Chik - JE HIG, again differing from rubella virus, which was practically as good with rooster as with goose erythrocytes. Sheep erythrocytes with rubella virus gave incomplete reactions in HIG. Sensitization of erythrocytes with JE - Chik viruses is better, when borate-phosphate, - not phosphate buffer, is used. Optimum pH values for both viruses should be regarded. Sucrose-acetone, freon - borate-saline antigens from infected mice brain may be used for HIG with Chik - JE viruses.
Imprenta: Archives of Virology, v. 64, n. 4, p. 339-347, 1980
Identificador do objeto digital: 10.1007/BF01320619
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology
Data de publicação: 1980
