Autor(es)Bréhin Anne-Claire, Rubrecht Laetitia, Navarro-Sanchez Martha Erika, Maréchal Valérie, Frenkiel Marie-Pascale, Lapalud Priscilla, Laune Daniel, Sall Amadou Alpha, Desprès Philippe

ResumoChikungunya fever is an arbovirosis of major impact in public health in Asia - Africa. Chikungunya (CHIK) virus is member of the genus Alphavirus - belongs to the Semliki Forest (SF) antigenic complex. We describe for the first time a panel of monoclonal antibodies (MAbs) reactive to CHIK envelope E2 glycoprotein. For the screening of E2-specific MAbs, we expressed a recombinant soluble CHIK E2 protein in Drosophila S2 cells. Analyzed by immunological methods, MAbs 3C3, 3E4, - 8A4 were selected on the basis of their reactivity. Their epitopes are located to the outer surface of CHIK virion. These MAbs have no cross reactivity with related members of SF antigenic complex with the notable exception of Igbo-Ora virus. Anti-CHIK E2 MAbs 3C3, 3E4, - 8A4 should be helpful for studying the biology of CHIK virus - pathogenesis of disease. The combination of 8A4 - 3E4 is suitable for developing a specific antigen-capture ELISA.

Palavras-ChaveChikungunya virus; Alphaviruses; Envelope E2 glycoprotein; Recombinant E2 antigen; Monoclonal antibodies; Indirect ELISA; Antigen-capture ELISA; Immunofluorescence assay; Immunoblot analysis; Viral diagnosis

ImprentaVirology, v. 371, n. 1, p. 185-195, 2008

DescritoresChikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Protein synthesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de Publicação:2008