Autor(es)Li Yong-Gang, Siripanyaphinyo Uamporn, Tumkosit Uranan, Noranate Nitchakarn, A-Nuegoonpipat Atchareeya, Pan Yang,Kameoka Masanori, Kurosu Takeshi, Ikuta Kazuyoshi, Takeda Naokazu, Anantapreecha Surapee

ResumoDouble-str-ed RNA (dsRNA) - its mimic, polyinosinic acid: polycytidylic acid [Poly (I:C)], are recognized by toll-like receptor 3 (TLR3) - induce interferon (IFN)-? in many cell types. Poly (I:C) is the most potent IFN inducer. In in vivo mouse studies, intraperitoneal injection of Poly (I:C) elicited IFN-?/? production - natural killer (NK) cells activation. The TLR3 pathway is suggested to contribute to innate immune responses against many viruses, including influenza virus, respiratory syncytial virus, herpes simplex virus 2, - murine cytomegalovirus. In Chikungunya virus (CHIKV) infection, the viruses are cleared within 7-10 days postinfection before adaptive immune responses emerge. The innate immune response is important for CHIKV clearance. The effects of Poly (I:C) on the replication of CHIKV in human bronchial epithelial cells, BEAS-2B, were studied. Poly (I:C) suppressed cytopathic effects (CPE) induced by CHIKV infection in BEAS-2B cells in the presence of Poly (I:C) - inhibited the replication of CHIKV in the cells. The virus titers of Poly (I:C)-treated cells were much lower compared with those of untreated cells. CHIKV infection - Poly (I:C) treatment of BEAS-2B cells induced the production of IFN-? - increased the expression of anti-viral genes, including IFN-?, IFN-?, MxA, - OAS. Both Poly (I:C) - CHIKV infection upregulate the expression of TLR3 in BEAS-2B cells. CHIKV is sensitive to innate immune response induced by Poly (I:C). The inhibition of CHIKV replication by Poly (I:C) may be through the induction of TLR3, which triggers the production of IFNs - other anti-viral genes. The innate immune response is important to clear CHIKV in infected cells.

Palavras-ChaveChikungunya virus; Poly (I:C); BEAS-2B cells; TLR3

ImprentaVirology Journal, v. 9, p. 114, 2012

DescritoresChikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology

Data de Publicação:2012