Phenotypic and functional analyses of NK and NKT-like populations during the early stages of chikungunya infection
Autor(es): Thanapati Subrat, Das Rumki, Tripathy Anuradha S
Resumo: The aim of this study was to characterize NK (CD56(+)CD3(-)) - NKT-like cell (CD56(+)CD3(+)) responses early after chikungunya infection. Expression profiling - functional analysis of T/NK/NKT-like cells were performed on samples from 56 acute - 31 convalescent chikungunya patients - 56 control individuals. The percentages of NK cells were high in both patient groups, whereas NKT-like cell percentages were high only in the convalescent group. The percentages of NKp30(+)CD3(-)CD56(+), NKp30(+)CD3(+)CD56(+), CD244(+)CD3(-)CD56(+), - CD244(+)CD3(+)CD56(+)cells were high, whereas the percentages of NKG2D(+)CD3(-)CD56(+) - NKG2D(+)CD3(+)CD56(+)cells were low in both patient groups. The percentages of NKp44(+)CD3(-)CD56(+) cells were high in both patient groups, whereas the percentages of NKp44(+)CD3(+)CD56(+) cells were higher in the acute group than in convalescent - control groups. The percentages of NKp46(+)CD3(-)CD56(+) cells were high in both patient groups. Higher percentages of perforin(+)CD3(-)CD56(+) - perforin(+)CD3(+)CD56(+) cells were observed in acute - convalescent patients, respectively. Higher cytotoxic activity was observed in acute patients than in controls. IFN-? expression on NK cells of convalescent patients - on NKT-like cells of both patient groups was indicative of the regulatory role of NK - NKT-like cells. Collectively, these data showed that higher expression of activating receptors on NK/NKT-like cells - perforin(+) NK cells in acute patients could be responsible for increased cytotoxicity. The observed expression of perforin(+) NK cells in the acute phase - IFN-?(+) NKT-like cells in the subsequent convalescent stage showed that NK/NKT-like cells mount an early - efficient response to chikungunya virus. Further study of the molecular mechanisms that limit viral dissemination/establishment of chronic disease will aid in underst-ing how NK/NKT-like cells control chikungunya infection.
Palavras-Chave: NK cell; NKT-like cell; Chikungunya; Cytotoxicity; Perforin
Imprenta: Frontiers in Microbiology, v. 6, p. 895, 2015
Identificador do objeto digital: 10.3389/fmicb.2015.00895
Descritores: Chikungunya virus - Cell ; Chikungunya Virus - Virus
Data de publicação: 2015
