pH-Dependent entry of Chikungunya virus fusion into mosquito cells
Autor(es): Nuckols John T, McAuley Alexander J, Huang Yan-Jang S, Horne Kate M, Higgs Stephen, Davey Robert A, Vanlandingham Dana L
Resumo: Millions of human infections caused by arthropod-borne pathogens are initiated by the feeding of an infected mosquito on a vertebrate. However, interactions between the viruses - the mosquito vector, which facilitates successful infection - transmission of virus to a subsequent vertebrate host, are still not fully understood. Here we describe early chikungunya virus (CHIKV) infectious events in cells derived from one of the most important CHIKV vectors, Aedes albopictus. We demonstrated that CHIKV infection of mosquito cells depended on acidification of the endosome as indicated by significant inhibition following prophylactic treatment with the lysosomotropic drugs chloroquine, ammonium chloride, - monensin, which is consistent with observations in mammalian cells. While all three agents inhibited CHIKV infection in C6/36 cells, ammonium chloride was less toxic to cells than the other agents. The observation of similar mechanisms for inhibition of CHIKV infection in mosquito - mammalian cell lines suggests that conserved entry pathways are utilized by CHIKV for vertebrate - invertebrate cell types.
Palavras-Chave: Chikungunya virus; Lysosomotropic compounds; Virus entry
Imprenta: Virology Journal, v. 11, p. 215, 2014
Identificador do objeto digital: 10.1186/s12985-014-0215-y
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Vaccine ; Chikungunya virus - Immunology
Data de publicação: 2014
