Autor(es): Madani Tariq A, Abuelzein El-Tayeb M E, Al-Bar Hussein M S, Azhar Esam I, Kao Moujahed, Alshoeb Haj O, Bamoosa Alabd R

Resumo: Investigations were conducted by the authors to explore an outbreak of viral hemorrhagic fever (VHF) reported in 2010 from Al-Mukalla city, the capital of Hadramout in Yemen. From 15-17 June 2010, the outbreak investigation period, specimens were obtained within 7 days after onset of illness of 18 acutely ill patients hospitalized with VHF - 15 household asymptomatic contacts of 6 acute cases. Additionally, 189 stored sera taken from acutely ill patients with suspected VHF hospitalized in the preceding 12 months were obtained from the Ministry of Health of Yemen. Thus, a total of 222 human specimens were collected; 207 specimens from acute cases - 15 specimens from contacts. All samples were tested with RT-PCR for dengue (DENV), Alkhumra (ALKV), Rift Valley Fever (RVFV), Yellow Fever (YFV), - Chikungunya (CHIKV) viruses. Samples were also tested for DENV IgM, IgG, - NS1-antigen. Medical records of patients were reviewed - demographic, clinical, - laboratory data was collected. Of 207 patients tested, 181 (87.4%) patients were confirmed to have acute dengue with positive dengue NS1-antigen (97 patients, 46.9%) -/or IgM (163 patients, 78.7%). Of the 181 patients with confirmed dengue, 100 (55.2%) patients were IgG-positive. DENV RNA was detected in 2 (1%) patients with acute symptoms; both samples were molecularly typed as DENV type 3. No other VHF viruses were detected. For the 15 contacts tested, RT-PCR tests for the five viruses were negative, one contact was dengue IgM positive, - another one was dengue IgG positive. Of the 181 confirmed dengue patients, 120 (66.3%) patients were males - the median age was 24 years. The most common manifestations included fever (100%), headache (94.5%), backache (93.4%), malaise (88.4%), arthralgia (85.1%), myalgia (82.3%), bone pain (77.9%), - leukopenia (76.2%). Two (1.1%) patients died. DENV-3 was confirmed to be the cause of an outbreak of VHF in Al-Mukalla. It is important to use both IgM - NS1-antigen tests to confirm acute dengue particularly under the adverse field conditions, where proper storage - transportation of specimens are missing, which substantially reduce the sensitivity of the RT-PCR for detecting DENV RNA.

Palavras-Chave: Dengue virus type 3; Viral hemorrhagic fever; Outbreak; Al-Mukalla; Yemen

Imprenta: BMC Infectious Diseases, v. 13, p. 136, 2013

Identificador do objeto digital: 10.1186/1471-2334-13-136

Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemiology ; Chikungunya virus - Public health

Data de publicação: 2013

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