Autor(es)Mattar Salim, Miranda Jorge, Pinzon Hernando, Tique Vanesa, Bolanos Amada, Aponte Jose, Arrieta German, Gonzalez Marco, Barrios Katerine, Contreras Hector, Alvarez Jaime, Aleman Ader

ResumoThe Caribbean area of Colombia has been severely affected by a Chikungunya virus (CHIKV) outbreak since 2014. The study was carried out on 100 patients during a fever outbreak from August to September 2014 in two small rural villages in the northern Caribbean area of Colombia. The molecular assays performed by reverse transcription polymerase chain reaction (RT-PCR) on acute patient sera were collected within one to five days of the appearance of symptoms. Sequence analyses were carried out based on phylogenetic analyses of genes NS1 - E2. For serological assays, 49 (49%) patients at ? 6 days of disease onset were tested with NovaLisa Chikungunya IgG/IgM µ-capture enzyme-linked immunosorbent assay (ELISA). The main signs or symptoms associated with Chikungunya infection were arthralgia of the lower limbs (96%), fever (91%), arthralgia of the upper limbs (85%), rash (64%), - headache (57%). Ninety-four percent (46/49) of patients were positive for either IgM or IgG; the remaining three (6%) patients were seronegative. Viral loads were detected in 25 patients. Based on phylogenetic analysis of NS1 - E2, the characterization of the Colombian CHIKV indicated that it was a strain closely related to the British Virgin Isl-s strain - to the Asian genotype. This study shows the phylogenetic - clinical description of CHIKV in Colombia. The main symptoms shown were: arthralgia, fever, - rash. CHIKV sequences detected in Colombian patients were within the Asian genotype - closely related to the British Virgin Isl-s strain.

ImprentaJournal of Infection in Developing Countries, v. 9, n. 10, p. 1126-1132, 2015

DescritoresChikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever

Data de Publicação:2015