Outbreak of Chikungunya virus in the north Caribbean area of Colombia: Clinical presentation and phylogenetic analysis
Autor(es): Mattar Salim, Miranda Jorge, Pinzon Hernando, Tique Vanesa, Bolanos Amada, Aponte Jose, Arrieta German, Gonzalez Marco, Barrios Katerine, Contreras Hector, Alvarez Jaime, Aleman Ader
Resumo: The Caribbean area of Colombia has been severely affected by a Chikungunya virus (CHIKV) outbreak since 2014. The study was carried out on 100 patients during a fever outbreak from August to September 2014 in two small rural villages in the northern Caribbean area of Colombia. The molecular assays performed by reverse transcription polymerase chain reaction (RT-PCR) on acute patient sera were collected within one to five days of the appearance of symptoms. Sequence analyses were carried out based on phylogenetic analyses of genes NS1 - E2. For serological assays, 49 (49%) patients at ? 6 days of disease onset were tested with NovaLisa Chikungunya IgG/IgM µ-capture enzyme-linked immunosorbent assay (ELISA). The main signs or symptoms associated with Chikungunya infection were arthralgia of the lower limbs (96%), fever (91%), arthralgia of the upper limbs (85%), rash (64%), - headache (57%). Ninety-four percent (46/49) of patients were positive for either IgM or IgG; the remaining three (6%) patients were seronegative. Viral loads were detected in 25 patients. Based on phylogenetic analysis of NS1 - E2, the characterization of the Colombian CHIKV indicated that it was a strain closely related to the British Virgin Isl-s strain - to the Asian genotype. This study shows the phylogenetic - clinical description of CHIKV in Colombia. The main symptoms shown were: arthralgia, fever, - rash. CHIKV sequences detected in Colombian patients were within the Asian genotype - closely related to the British Virgin Isl-s strain.
Imprenta: Journal of Infection in Developing Countries, v. 9, n. 10, p. 1126-1132, 2015
Identificador do objeto digital: 10.3855/jidc.6670
Descritores: Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever
Data de publicação: 2015
