Osteoclastogenesis induced by CHIKV-infected fibroblast-like synoviocytes: A possible interplay between synoviocytes and monocytes/macrophages in CHIKV-induced arthralgia/arthritis
Autor(es): Phuklia Weerawat, Kasisith Jitra, Modhiran Naphak, Rodpai Ekkarat, Thannagith Montri, Thongsakulprasert Tipparat, Smith Duncan R, Ubol Sukathida
Resumo: Fibroblast-like synoviocytes are known to migrate from joint to joint - are proposed to be one of the key players in the inflammatory cascade amplification in rheumatoid arthritis patients. In the recent CHIKV epidemic, patients developed arthritis-like syndrome - the synoviocyte is one of the suspected players in CHIKV-induced polyarthritis. Thus, to learn more on this syndrome, the responses of fibroblast-like synoviocytes to chikungunya virus (CHIKV) infection, - the interaction between CHIKV-infected synoviocytes - phagocytes, were investigated. Primary human fibroblast-like synoviocyte (HFLS) cultures were infected with clinical isolates of CHIKV at an MOI of 0.001pfu/cell. Data indicated that HFLS are permissive to CHIKV replication, generating peak titers of 10(5)-10(6)pfu/ml. Interestingly, CHIKV-infected HFLS cultures secreted mainly the mediators that are responsible for phagocytes recruitment - differentiation (RANKL, IL-6, IL-8 - MCP-1) but not arthritogenic mediators (TNF-?, IL-1?, MMP-1, MMP-2 or MMP-13). The interaction between CHIKV-infected synoviocytes - phagocytes was studied using UV-irradiated, CHIKV-infected HFLS supernatant. Data revealed that supernatants from CHIKV-infected HFLS cultures not only induced migration of primary human monocytes, but also drove monocytes/macrophages into osteoclast-like cells. These differentiated osteoclast-like cells produced high levels of TNF-? - IL-6, principal mediators of arthritis. This data suggests a potential interplay between infected HFLS - recruiting phagocytes which may responsible for the arthralgia/arthritis in CHIKV-infected patients.
Palavras-Chave: Arthralgia/arthritis; Chikungunya; Fibroblast-like synoviocytes; Osteoclast
Imprenta: Virus Research, v. 177, n. 2, p. 179-188, 2013
Identificador do objeto digital: 10.1016/j.virusres.2013.08.011
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology
Data de publicação: 2013
