Autor(es): Konishi E, Hotta S

Resumo: The mechanism of the action of tannic acid (TA) Chikungunya virus (CHIKV) was investigated. Both infectivity - hemagglutination (HA) activity of CHIKV were reduced by treatment with TA in vitro. Aggregation of the TA-treated virus particles was observed by electron microscopy. The reaction was reversible, depending on the pH of the mixture. However, mere dilution of the TA-virus mixture or addition of other protein, such as bovine serum albumin, did not restore the lost infectivity. TA also suppressed the infectivity of RNA extracted from the virus - the HA activity of the viral membrane. The affinity between TA - CHIKV structural proteins (E1, E2, - C) was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in a discontinuous buffer system - the order of affinity was found to be C greater than E1 greater than E2. Specific conditions for binding of TA to each of the virus proteins were investigated.

Imprenta: Microbiology and Immunology, v. 24, n. 9, p. 847-859, 1980

Identificador do objeto digital: 10.1111/j.1348-0421.1980.tb02889.x

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya Virus - Virus

Data de publicação: 1980

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