Nonstructural protein 2 (nsP2) of Chikungunya virus (CHIKV) enhances protective immunity mediated by a CHIKV envelope protein expressing DNA Vaccine
Autor(es): Bao Huihui, Ramanathan Aarti A, Kawalakar Omkar, Sundaram Senthil G, Tingey Colleen, Bian Charoran B, Muruganandam Nagarajan, Vijayachari Paluru, Sardesai Niranjan Y, Weiner David B, Ugen Kenneth E, Muthumani Karuppiah
Resumo: Chikungunya virus (CHIKV) is an important emerging mosquito-borne alphavirus, indigenous to tropical Africa - Asia. It can cause epidemic fever - acute illness characterized by fever - arthralgias. The epidemic cycle of this infection is similar to dengue - urban yellow fever viral infections. The generation of an efficient vaccine against CHIKV is necessary to prevent -/or control the disease manifestations of the infection. In this report, we studied immune response against a CHIKV-envelope DNA vaccine (pEnv) - the role of the CHIKV nonstructural gene 2 (nsP2) as an adjuvant for the induction of protective immune responses in a relevant mouse challenge model. When injected with the CHIKV pEnv alone, 70% of the immunized mice survived CHIKV challenge, whereas when co-injected with pEnv+pnsP2, 90% of the mice survived viral challenge. Mice also exhibited a delayed onset signs of illness, - a marked decrease in morbidity, suggesting a nsP2 mediated adjuvant effect. Co-injection of the pnsP2 adjuvant with pEnv also qualitatively - quantitatively increased antigen specific neutralizing antibody responses compared to vaccination with pEnv alone. In sum, these novel data imply that the addition of nsP2 to the pEnv vaccine enhances anti-CHIKV-Env immune responses - maybe useful to include in future CHIKV clinical vaccination strategies.
Imprenta: Viral Immunology, v. 26, n. 1, p. 75-83, 2013
Identificador do objeto digital: 10.1089/vim.2012.0061
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cytopathology ; Chikungunya virus - DNA ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Immune response ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2013
