Next generation sequencing reveals regulation of distinct Aedes microRNAs during Chikungunya virus development
Autor(es): Shrinet Jatin, Jain Shanu, Jain Jaspreet, Bhatnagar Raj K, Sunil Sujatha
Resumo: Application of genomics - Next Generation sequencing has led to the identification of new class of cellular functional molecules, namely, small RNAs. Of the several classes of ncRNAs (non-coding RNA), microRNAs have been demonstrated to exert determinative influence on various cellular processes. It is becoming abundantly clear that host/vector/pathogen encoded microRNAs impact eventual pathogenesis. In this context, the participation of vector based microRNAs in disease transmission - pathogen development is being investigated intensively. A few studies have highlighted the role of vector encoded microRNAs in pathogen infection. We conducted this study to evaluate the role of host miRNAs upon CHIKV (Chikungunya Virus) infection in an important vector, Aedes albopictus. We identified 88 - 79 known miRNAs in uninfected - CHIKV infected Ae. albopictus Singh's cell line respectively. We further identified nine novel miRNAs in Ae. albopictus. Comparison of the two libraries revealed differential expression of 77 common miRNAs between them. CHIKV infection specifically altered the miRNA profile of a specific set of eight miRNAs. Putative targets of these regulated miRNAs were identified - classified into their pathways. In our study we have identified - described the profiles of various miRNAs upon CHIKV infection in Ae. albopictus. This investigation provides an insight about cellular modification by miRNAs during CHIKV infection - the results provide leads for identifying potential c-idates for vector based antiviral strategies.
Imprenta: PLoS Neglected Tropical Diseases, v. 8, n. 1, p. e2616, 2014
Identificador do objeto digital: 10.1371/journal.pntd.0002616
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Public health
Data de publicação: 2014
