Autor(es): Kumar Shiril, Jaffar-B-jee Marie-Christine, Giry Claude, Connen de Kerillis Léa, Merits Andres, Gasque Philippe, Hoarau Jean-Jacques

Resumo: Infection with Chikungunya alphavirus (CHIKV) can cause severe arthralgia - chronic arthritis in humans with persistence of the virus in perivascular macrophages of the synovial membrane by mechanisms largely ill-characterized. We herein analysed the innate immune response (cytokine - programmed cell death) of RAW264.7 mouse macrophages following CHIKV infection. We found that the infection was restrained to a small percentage of cells - was not associated with a robust type I IFN innate immune response (IFN-?4 - ISG56). TNF-?, IL-6 - GM-CSF expression were upregulated while IFN-?, IL-1?, IL-2, IL-4, IL-5, IL-10 or IL-17 expression could not be evidenced prior to - after CHIKV exposure. Although CHIKV is known to drive apoptosis in many cell types, we found no canonical signs of programmed cell death (cleaved caspase-3, -9) in infected RAW264.7 cells. These data argue for the capacity of CHIKV to infect - drive a specific innate immune response in RAW264.7 macrophage cell which seems to be polarized to assist viral persistence through the control of apoptosis - IFN signalling.

Palavras-Chave: Chikungunya virus; Macrophage; Apoptosis; Viral persistence; Inflammation

Imprenta: Virology Journal, v. 9, p. 313, 2012

Identificador do objeto digital: 10.1186/1743-422X-9-313

Descritores: Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - Immunopathology ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya virus - Real Time PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology

Data de publicação: 2012

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